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Registro Completo |
Biblioteca(s): |
Embrapa Trigo. |
Data corrente: |
16/06/2021 |
Data da última atualização: |
30/11/2021 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVA, V. M. da; MARQUES, L. U.; KLAESSENER, L. A.; ABEL, F. de S.; BIASUZ, A. L.; NASCIMENTO JUNIOR, A. do. |
Afiliação: |
VÍCTOR MARQUES DA SILVA, Estudante de Agronomia, Universidade de Passo Fundo-UPF, Passo Fundo, RS. Estagiário da Embrapa Trigo, Passo Fundo, RS.; LUCAS UGHINI MARQUES, Estudante de Agronomia, Universidade de Passo Fundo-UPF, Passo Fundo, RS. Estagiário da Embrapa Trigo, Passo Fundo, RS.; LUCAS ALAN KLAESSENER, 1)Estudante de Agronomia, Universidade de Passo Fundo-UPF, Passo Fundo, RS. Estagiário da Embrapa Trigo, Passo Fundo, RS; FERNANDO DE SOUZA ABEL, Estudante de Agronomia, Universidade de Passo Fundo-UPF, Passo Fundo, RS. Estagiário da Embrapa Trigo, Passo Fundo, RS.; ANDRÉ LUIZ BIASUZ, Estudante de Agronomia, Universidade de Passo Fundo-UPF, Passo Fundo, RS. Estagiário da Embrapa Trigo, Passo Fundo, RS.; ALFREDO DO NASCIMENTO JUNIOR, CNPT. |
Título: |
Melhoramento genético de aveia forrageira na Embrapa Trigo. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 15.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 12., 2020, Passo Fundo. Resumos... Brasília, DF: Embrapa, p. 27, 2021. |
Idioma: |
Português |
Conteúdo: |
A aveia é uma importante cultura de inverno no sul do País, podendo ser utilizada para a produção de grãos ou então para produção de matéria seca de plantas para uso forrageiro ou cobertura de solo. |
Palavras-Chave: |
Alimentação animal. |
Thesagro: |
Avena Sativa; Avena Strigosa. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/223824/1/Silva-p27-MIC-MPG-2020-completo.pdf
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Marc: |
LEADER 00918nam a2200205 a 4500 001 2132350 005 2021-11-30 008 2021 bl uuuu u00u1 u #d 100 1 $aSILVA, V. M. da 245 $aMelhoramento genético de aveia forrageira na Embrapa Trigo.$h[electronic resource] 260 $aIn: MOSTRA DE INICIAÇÃO CIENTÍFICA, 15.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 12., 2020, Passo Fundo. Resumos... Brasília, DF: Embrapa, p. 27$c2021 520 $aA aveia é uma importante cultura de inverno no sul do País, podendo ser utilizada para a produção de grãos ou então para produção de matéria seca de plantas para uso forrageiro ou cobertura de solo. 650 $aAvena Sativa 650 $aAvena Strigosa 653 $aAlimentação animal 700 1 $aMARQUES, L. U. 700 1 $aKLAESSENER, L. A. 700 1 $aABEL, F. de S. 700 1 $aBIASUZ, A. L. 700 1 $aNASCIMENTO JUNIOR, A. do
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Registro original: |
Embrapa Trigo (CNPT) |
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Registro Completo
Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
16/11/2018 |
Data da última atualização: |
11/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
ALESSI, A. M.; BIRD, S. M.; OATES, N. C.; LI, Y.; DOWLE, A. A.; NOVOTNY, E. H.; AZEVEDO, E. R. de; BENNETT, J. P.; POLIKARPOV, I.; YOUNG, J. P. W.; MCQUEEN-MASON, S. J.; BRUCE, N. C. |
Afiliação: |
ANNA M. ALESSI, UNIVERSITY OF YORK; SUSANNAH M. BIRD, UNIVERSITY OF YORK; NICOLA C. OATES, UNIVERSITY OF YORK; YI LI, UNIVERSITY OF YORK; ADAM A. DOWLE, UNIVERSITY OF YORK; ETELVINO HENRIQUE NOVOTNY, CNPS; EDUARDO R. DE AZEVEDO, USP; JOSEPH P. BENNETT, UNIVERSITY OF YORK; IGOR POLIKARPOV, USP; J. PETER W. YOUNG, UNIVERSITY OF YORK; SIMON J. MCQUEEN-MASON, UNIVERSITY OF YORK; NEIL C. BRUCE, UNIVERSITY OF YORK. |
Título: |
Defining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Biotechnology for Biofuels, v. 11, article 166, 2018. |
DOI: |
https://doi.org/10.1186/s13068-018-1164-2 |
Idioma: |
Inglês |
Conteúdo: |
Background: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation. MenosBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacau... Mostrar Tudo |
Palavras-Chave: |
CAZy; Metasecretome. |
Thesaurus NAL: |
Lignocellulose. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186133/1/2018-044.pdf
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Marc: |
LEADER 03644naa a2200301 a 4500 001 2099501 005 2021-11-11 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1186/s13068-018-1164-2$2DOI 100 1 $aALESSI, A. M. 245 $aDefining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies.$h[electronic resource] 260 $c2018 520 $aBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation. 650 $aLignocellulose 653 $aCAZy 653 $aMetasecretome 700 1 $aBIRD, S. M. 700 1 $aOATES, N. C. 700 1 $aLI, Y. 700 1 $aDOWLE, A. A. 700 1 $aNOVOTNY, E. H. 700 1 $aAZEVEDO, E. R. de 700 1 $aBENNETT, J. P. 700 1 $aPOLIKARPOV, I. 700 1 $aYOUNG, J. P. W. 700 1 $aMCQUEEN-MASON, S. J. 700 1 $aBRUCE, N. C. 773 $tBiotechnology for Biofuels$gv. 11, article 166, 2018.
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